Pure non-dioxin-like PCB congeners suppress induction of AhR-dependent endpoints in rat liver cells

0456569 - BFÚ 2016 RIV DE eng J - Journal Article
Brenerová, P. - Hamers, T. - Kamstra, J.H. - Vondráček, Jan - Střapacova, S. - Andersson, P.L. - Machala, M.
Pure non-dioxin-like PCB congeners suppress induction of AhR-dependent endpoints in rat liver cells.
Environmental Science and Pollution Research. Roč. 23, č. 3 (2016), s. 2099-2107. ISSN 0944-1344. E-ISSN 1614-7499
R&D Projects: GA ČR(CZ) GBP503/12/G147
Institutional support: RVO:68081707
Keywords : NDL-PCBs * Aryl hydrocarbon receptor * DR-CALUX (R) assay
Subject RIV: BO - Biophysics
Impact factor: 2.741, year: 2016

The relative potencies of non-ortho-substituted coplanar polychlorinated biphenyl (PCB) congeners to activate the aryl hydrocarbon receptor (AhR) and to cause the AhR-dependent toxic events are essential for their risk assessment. Since some studies suggested that abundant non-dioxin-like PCB congeners (NDL-PCBs) may alter the AhR activation by PCB mixtures and possibly cause non-additive effects, we evaluated potential suppressive effects of NDL-PCBs on AhR activation, using a series of 24 highly purified NDL-PCBs. We investigated their impact on the model AhR agonist-induced luciferase reporter gene expression in rat hepatoma cells and on induction of CYP1A1/1B1 mRNAs and deregulation of AhR-dependent cell proliferation in rat liver epithelial cells. PCBs 128, 138, and 170 significantly suppressed AhR activation (with IC50 values from 1.4 to 5.6 mu M), followed by PCBs 28, 47, 52, and 180; additionally, PCBs 122, 153, and 168 showed low but still significant potency to reduce luciferase activity.
Permanent Link: http://hdl.handle.net/11104/0257127