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Analytical Separation Science
- 1.0456541 - ÚOCHB 2016 DE eng M - Monography Chapter
Kašička, Václav
Capillary electromigration techniques: Capillary electrophoresis.
Analytical Separation Science. Vol. 2. Weinheim: Wiley, 2015 - (Anderson, J.; Berthod, A.; Pino Estévez, V.; Stalcup, A.), s. 505-529. ISBN 978-3-527-33374-5
R&D Projects: GA ČR(CZ) GAP206/12/0453; GA ČR(CZ) GA13-17224S
Institutional support: RVO:61388963
Keywords : electrophoretic mobility * electroosmotic flow * analysis * ionogenic compounds
Subject RIV: CB - Analytical Chemistry, Separation
This chapter provides a brief introduction to capillary electrophoresis (CE) – a modern high-performance electromigration method. First, basic concepts of electrophoresis in general, such as ionic, effective and limiting electrophoretic mobilities, their concentration, and pH dependencies are presented, and the concomitant electrokinetic phenomenon – electroosmotic flow – is introduced. Second, the separation principle and the basic experimental setup of CE are shown and the advantages and limitations of CE are reported. Further, the dispersion effects in CE and the ways of minimization of their detrimental effects on the separation efficiency of CE are described. Finally, the high application potential of CE for analysis, microscale isolation, and physicochemical and biochemical characterization of a large spectrum of all types of ionogenic compounds and (bio)particles is emphasized. For more detailed studies of CE and other capillary electromigration methods, many literature references are given.
Permanent Link: http://hdl.handle.net/11104/0257065
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