Exacerbation of substrate toxicity by IPTG in Escherichia coli BL21 (DE3) carrying a synthetic metabolic pathway

0456331 - BFÚ 2016 RIV GB eng J - Journal Article
Dvořák, P. - Chrást, L. - Nikel, P. - Fedr, Radek - Souček, Karel - Sedláčková, M. - Chaloupková, R. - Lorenzo, V. - Prokop, Z. - Damborský, J.
Exacerbation of substrate toxicity by IPTG in Escherichia coli BL21 (DE3) carrying a synthetic metabolic pathway.
Microbial Cell Factories. Roč. 14, č. 201 (2015). E-ISSN 1475-2859
Institutional support: RVO:68081707
Keywords : Metabolic burden * Substrate toxicity * Escherichia coli
Subject RIV: BO - Biophysics
Impact factor: 3.744, year: 2015

Background: Heterologous expression systems based on promoters inducible with isopropyl-beta-D-1-thiogalactopyranoside (IPTG), e.g., Escherichia coli BL21 (DE3) and cognate LacI(Q)/P-lacUV5-T7 vectors, are commonly used for production of recombinant proteins and metabolic pathways. The applicability of such cell factories is limited by the complex physiological burden imposed by overexpression of the exogenous genes during a bioprocess. This burden originates from a combination of stresses that may include competition for the expression machinery, side-reactions due to the activity of the recombinant proteins, or the toxicity of their substrates, products and intermediates. However, the physiological impact of IPTG-induced conditional expression on the recombinant host under such harsh conditions is often overlooked.
Permanent Link: http://hdl.handle.net/11104/0256895