Label-free electrochemical detection of singlet oxygen protein damage

0456295 - BFÚ 2016 RIV GB eng J - Journal Article
Vargová, Veronika - Gimenez, R.E. - Černocká, Hana - Trujillo, D.C. - Tulli, F. - Zanini, V.I.P. - Paleček, Emil - Borsarelli, C.D. - Ostatná, Veronika … Total 10 authors
Label-free electrochemical detection of singlet oxygen protein damage.
Electrochimica acta. Roč. 187, JAN 2016 (2016), s. 662-669. ISSN 0013-4686. E-ISSN 1873-3859
R&D Projects: GA ČR GA13-00956S
Institutional support: RVO:68081707
Keywords : singlet oxygen protein damage * surface-attached protein stability * mercury and carbon electrodes
Subject RIV: BO - Biophysics
Impact factor: 4.798, year: 2016

Oxidative damage of proteins results in changes of their structures and functions. In this work, the singlet oxygen (O-1(2))-mediated oxidation of bovine serum albumin (BSA) and urease by blue-light photosensitization of the tris(2,2'-bipyridine)ruthenium(II) cation [Ru(bpy)(3)](2+) was studied by square wave voltammetry at glassy carbon electrode and by constant current chronopotentiometry at mercury electrode. Small changes in voltammetric oxidation Tyr and Trp peaks did not indicate significant changes in the BSA structure after photo-oxidation at carbon electrode. On the other hand chronopotentiometric peak H of BSA at HMDE increased during blue-light photosensitization, indicating that photo-oxidized BSA was more susceptible to the electric field-induced denaturation than non-oxidized native BSA. Similar results were obtained for urease, where enzymatic activity was also evaluated. The present results show the capability of label- and reagent-free electrochemical methods to detect oxidative changes in proteins. We believe that these methods will become important tools for detection of various protein damages. (C) 2015 Elsevier Ltd. All rights reserved.
Permanent Link: http://hdl.handle.net/11104/0256852