Comprehensive physiology and toxicology of ecdysogens – The metabolically activated porphyrin-ecdysteroid complexes in insects

0456116 - BC 2017 RIV US eng J - Journal Article
Sláma, Karel - Zhylitskaya, H.
Comprehensive physiology and toxicology of ecdysogens – The metabolically activated porphyrin-ecdysteroid complexes in insects.
Comparative Biochemistry and Physiology Part C. 181-182, March 31 (2016), s. 55-67. ISSN 1532-0456. E-ISSN 1878-1659
Institutional support: RVO:60077344
Keywords : ecdysone * 20-hydroxyecdysone (E20) * ecdysteroids (Ecds)
Subject RIV: ED - Physiology
Impact factor: 2.416, year: 2016
http://www.sciencedirect.com/science/article/pii/S1532045616300011

The polyhydroxylated derivatives of 6-keto,7-dehydrocholesterol (ecdysone, ecdysteroids, Ecd) are natural compounds widely distributed in plants. They exhibit strong anabolic, vitamin D-like, pharmacological effects in vertebrate animals and in the human body. In the larval stages of insects, injections of pure Ecd cause serious pathophysiological, "hyperecdysonic" syndromes associated with neuromuscular paralysis, premature cuticular apolysis and complete inhibition of ecdysis. Ecds do not penetrate insect cuticle. For this reason, all previous attempts to induce ecdysone responses by topical applications of Ecd failed. In this work, we tried to induce the topical effects of Ecd by preparation of more lipophilic complexes, with 2 or 4 molecules of 20-hydroxyecdysone (E20) attached to a relatively large nucleus of the porphyrin. The resulting porphyrin-E20 complexes (ecdysogens) have been subjected to standardised assays for ecdysone activity in the ligatured larvae ("dauerlarvae") of the greater waxmoth (Galleria mellonella). Similarly like the free E20 alone, porphyrin-E20 complexes had no effect when applied on the body surface or administered in the larval diet. When injected, however, they exhibited delayed effects, but the adverse ("hyperecdysonic") pathophysiological syndromes were reduced or abolished. It is concluded, therefore, that the replacement of pathophysiological, precocious or "hyperecdysonic" moults by the larval-pupal transformation, was due to successive metabolic liberation of the biologically active, free E20 from the porphyrin-E20 complex. The biological status of Ecd does not agree with their definition as the prothoracic gland (PG) hormone of insects, nor with the assumptions about a growth hormone of plants. A possibility that the most important status of Ecd may depend on the pharmacological properties of a sterolic D6 vitamin has been discussed.
Permanent Link: http://hdl.handle.net/11104/0256715