Immunomodulatory Potency of Microcystin, an Important Water-Polluting Cyanobacterial Toxin

0455883 - BFÚ 2016 RIV US eng J - Journal Article
Adamovský, O. - Moosová, Z. - Pekarová, Michaela - Basu, A. - Babica, P. - Šindlerová, Lenka - Kubala, Lukáš - Blaha, L.
Immunomodulatory Potency of Microcystin, an Important Water-Polluting Cyanobacterial Toxin.
Environmental Science and Technology. Roč. 49, č. 20 (2015), s. 12457-12464. ISSN 0013-936X. E-ISSN 1520-5851
Institutional support: RVO:68081707
Keywords : NF-KAPPA-B * NECROSIS-FACTOR-ALPHA * TOLL-LIKE RECEPTORS
Subject RIV: BO - Biophysics
Impact factor: 5.393, year: 2015

Microcystins (MCs) are primarily hepatotoxins produced by cyanobacteria and are responsible for intoxication in humans and animals. There are many incidents of chronic exposure to MCs, which have been attributed to the inappropriate treatment of water supplies or contaminated food. Using RAW 264.7 macrophages, we showed the potency of microcystin-LR (MC-LR) to stimulate production of pro-inflammatory cytokines (tumor necrosis factor alpha and interleukin-6) as a consequence of fast nuclear factor kappa B and nitrogen-activated protein kinase activation. In contrast to other studies, the observed effects were not attributed to the intracellular inhibition of protein phosphatases 1/2A due to lack of specific transmembrane transporters for MCs. However, the MC-LR-induced activation of macrophages was effectively inhibited by a specific peptide that blocks signaling of receptors, which play a pivotal role in the innate immune responses.
Permanent Link: http://hdl.handle.net/11104/0256494