Anaerobic in situ biodegradation of TNT using whey as an electron donor: a case study

0455608 - MBÚ 2016 RIV NL eng J - Journal Article
Innemanová, P. - Velebová, R. - Filipová, Alena - Čvančarová, Monika - Pokorný, P. - Němeček, J. - Cajthaml, Tomáš
Anaerobic in situ biodegradation of TNT using whey as an electron donor: a case study.
New Biotechnology. Roč. 32, č. 6 (2015), s. 701-709. ISSN 1871-6784. E-ISSN 1876-4347
Institutional support: RVO:61388971
Keywords : CONTAMINATED SOIL * BIOTRANSFORMATION * BIOREMEDIATION
Subject RIV: EE - Microbiology, Virology
Impact factor: 3.199, year: 2015

Contamination by 2,4,6-trinitrotoluene (TNT), an explosive extensively used by the military, represents a serious environmental problem. In this study, whey has been selected as the most technologically and economically suitable primary substrate for anaerobic in situ biodegradation of TNT. Under laboratory conditions, various additions of whey, molasses, acetate and activated sludge as an inoculant were tested and the process was monitored using numerous chemical analyses including phospholipid fatty acid analysis. The addition of whey resulted in the removal of more than 90% of the TNT in real contaminated soil (7 mg kg(-1) and 12 mg kg(-1) of TNT). The final bioremediation strategy was suggested on the basis of the laboratory results and tested under real conditions at a TNT contaminated site in the Czech Republic. During the pilot test, three repeated injections of whey suspension into the sandy aquifer were performed over a 10-month period. In total, approximately 5 m(3) of whey were used.
Permanent Link: http://hdl.handle.net/11104/0256219