Assessment of degradation potential of aliphatic hydrocarbons by autochthonous filamentous fungi from a historically polluted clay soil

0455607 - MBÚ 2016 RIV NL eng J - Journal Article
Covino, Stefano - D´Annibale, A. - Stazi, S. R. - Cajthaml, Tomáš - Čvančarová, Monika - Stella, Tatiana - Petruccioli, M.
Assessment of degradation potential of aliphatic hydrocarbons by autochthonous filamentous fungi from a historically polluted clay soil.
Science of the Total Environment. Roč. 505, FEB 2015 (2015), s. 545-554. ISSN 0048-9697. E-ISSN 1879-1026
R&D Projects: GA MŠMT(CZ) EE2.3.30.0003; GA ČR GA13-28283S
Institutional support: RVO:61388971
Keywords : Mycoaugmentation * Pseudoallescheria sp * Oil-contaminated soil
Subject RIV: EE - Microbiology, Virology
Impact factor: 3.976, year: 2015

The present work was aimed at isolating and identifying the main members of the mycobiota of a clay soil historically contaminated by mid- and long-chain aliphatic hydrocarbons (AH) and to subsequently assess their hydrocarbon-degrading ability. All the isolates were Ascomycetes and, among them, the most interesting was Pseudoallescheria sp. 18A, which displayed both the ability to use AH as the sole carbon source and to profusely colonize a wheat straw:poplar wood chip (70:30, w/w) lignocellulosic mixture (LM) selected as the amendment for subsequent soil remediation microcosms. After a 60 d mycoaugmentation with Pseudoallescheria sp. of the aforementioned soil, mixed with the sterile LM (5:1 mass ratio), a 79.7% AH reduction and a significant detoxification, inferred by a drop in mortality of Folsomia candida from 90 to 24%, were observed. However, similar degradation and detoxification outcomes were found in the non-inoculated incubation control soil that had been amended with the sterile LM. This was due to the biostimulation exerted by the amendment on the resident microbiota, fungi in particular, the activity and density of which were low, instead, in the non-amended incubation control soil.
Permanent Link: http://hdl.handle.net/11104/0256196