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Longevity of U cells of differentiated yeast colonies grown on respiratory medium depends on active glycolysis

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    0455588 - MBÚ 2016 RIV US eng J - Journal Article
    Čáp, M. - Váchová, Libuše - Palková, Z.
    Longevity of U cells of differentiated yeast colonies grown on respiratory medium depends on active glycolysis.
    Cell Cycle. Roč. 14, č. 21 (2015), s. 3488-3497. ISSN 1538-4101. E-ISSN 1551-4005
    R&D Projects: GA ČR(CZ) GA15-08225S; GA MŠMT(CZ) ED1.1.00/02.0109
    Institutional support: RVO:61388971
    Keywords : enzymatic assays * fermentation * metabolic differentiation
    Subject RIV: EE - Microbiology, Virology
    Impact factor: 3.952, year: 2015

    Colonies of Saccharomyces cerevisiae laboratory strains pass through specific developmental phases when growing on solid respiratory medium. During entry into the so-called alkali phase, in which ammonia signaling is initiated, 2 prominent cell types are formed within the colonies: U cells in upper colony regions, which have a longevity phenotype and activate the expression of a large number of metabolic genes, and L cells in lower regions, which die more quickly and exhibit a starvation phenotype. Here, we performed a detailed analysis of the activities of enzymes of central carbon metabolism in lysates of both cell types and determined several fermentation end products, showing that previously reported expression differences are reflected in the different enzymatic capabilities of each cell type. Hence, U cells, despite being grown on respiratory medium, behave as fermenting cells, whereas L cells rely on respiratory metabolism and possess active gluconeogenesis.
    Permanent Link: http://hdl.handle.net/11104/0256186

     
     
Number of the records: 1  

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