Differentiation potential of neonatal neural stem/progenitor cells is affected by WNT signaling

0455570 - ÚMG 2016 RIV US eng A - Abstract
Kriška, Ján - Honsa, Pavel - Džamba, Dávid - Butenko, Olena - Tůmová, L. - Kořínek, Vladimír - Anděrová, Miroslava
Differentiation potential of neonatal neural stem/progenitor cells is affected by WNT signaling.
Journal of Neurochemistry. Wiley. Roč. 134, SI (2015), s. 181-181. ISSN 0022-3042. E-ISSN 1471-4159.
[25th Biennial Meeting of the International-Society-for-Neurochemistry Jointly with the 13th Meeting of the Asian-Pacific-Society-for-Neurochemistry in Conjuction with the 35th Meeting of the Australasian-Neuroscience-Society. 23.08.2015-27.08.2015, Cairns]
R&D Projects: GA ČR(CZ) GAP303/12/0855
Institutional support: RVO:68378041 ; RVO:68378050
Keywords : Wnt signaling * beta-catenin * neurogenesis
Subject RIV: EB - Genetics ; Molecular Biology

The canonical Wnt signaling pathway plays an important role in embryogenesis and in establishment of neurogenic niches. Studies using embryonic and neonatal mice suggested involvement of Wnt/beta-catenin signaling in proliferation and differentiation of neural progenitors. The effect of Wnt signaling on the differentiation potential of neonatal neural stem/progenitor cells (NS/PCs) and their membrane properties was studied eight days after the onset of in vitro differentiation. To manipulate Wnt signaling at different subcellular levels, three transgenic mouse strains were employed, which enabled either inhibition of the pathway at the nuclear or cell membrane level, or activation of Wnt signaling in the cytoplasm using the Cre-loxP system. To follow the fate of NS/PCs, the patch-clamp technique, immunocytochemistry, calcium imaging, Western blot, and quantitative RT-PCR (RT-qPCR) was utilized. Within differentiated cells we found three electrophysiologically and immunocytochemically distinct cell types, whose incidence was markedly affected by the Wnt signaling output. Activation of this pathway suppressed gliogenesis, and promoted differentiation of NS/PCs towards neuronal phenotype, while its inhibition led to the opposite effect. Moreover, Wnt signaling manipulation influenced the incidence of cells expressing voltage gated K+ and Na+ currents as well as cell responses to glutamate. The results indicate that the Wnt signaling pathway orchestrates neonatal NS/PCs differentiation towards cells with neuronal characteristics, which might be important for nervous tissue regeneration during central nervous system disorders
Permanent Link: http://hdl.handle.net/11104/0256175