Non-fouling biodegradable poly(.epsilon.-caprolactone) nanofibers for tissue engineering

0454747 - ÚMCH 2016 RIV DE eng J - Journal Article
Kostina, Nina Yu. - Pop-Georgievski, Ognen - Bachmann, M. - Neykova, Neda - Bruns, M. - Michálek, Jiří - Bastmeyer, M. - Rodriguez-Emmenegger, Cesar
Non-fouling biodegradable poly(.epsilon.-caprolactone) nanofibers for tissue engineering.
Macromolecular Bioscience. Roč. 16, č. 1 (2016), s. 83-94. ISSN 1616-5187. E-ISSN 1616-5195
R&D Projects: GA ČR(CZ) GJ15-09368Y; GA MŠMT(CZ) ED1.1.00/02.0109; GA MŠMT(CZ) LH13178
Institutional support: RVO:61389013 ; RVO:68378271
Keywords : antifouling * cell adhesion * nanofibers
Subject RIV: CD - Macromolecular Chemistry; BM - Solid Matter Physics ; Magnetism (FZU-D)
Impact factor: 3.238, year: 2016

Poly(ɛ-caprolactone) (PCL) nanofibers are very attractive materials for tissue engineering (TE) due to their degradability and structural similarity to the extracellular matrix (ECM). However, upon exposure to biological media, their surface is rapidly fouled by proteins and cells, which may lead to inflammation and foreign body reaction. In this study, an approach for the modification of PCL nanofibers to prevent protein fouling from biological fluids and subsequent cell adhesion is introduced. A biomimetic polydopamine (PDA) layer was deposited on the surface of the PCL nanofibers and four types of antifouling polymer brushes were grown by surface-initiated atom transfer radical polymerization (SI-ATRP) from initiator moieties covalently attached to the PDA layer. Cell adhesion was assessed with mouse embryonic fibroblasts (MEFs). MEFs rapidly adhered and formed cell–matrix adhesions (CMAs) with PCL and PCL-PDA nanofibers. Importantly, the nanofibers modified with antifouling polymer brushes were able to suppress non-specific protein adsorption and thereby cell adhesion.
Permanent Link: http://hdl.handle.net/11104/0256352