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Separation of Aeruginosin-865 from Cultivated Soil Cyanobacterium (Nostoc sp.) by Centrifugal Partition Chromatography combined with Gel Permeation Chromatography
- 1.0454171 - MBÚ 2016 RIV US eng J - Journal Article
Cheel, José - Minceva, M. - Urajová, Petra - Aslam, R. - Hrouzek, Pavel - Kopecký, Jiří
Separation of Aeruginosin-865 from Cultivated Soil Cyanobacterium (Nostoc sp.) by Centrifugal Partition Chromatography combined with Gel Permeation Chromatography.
Natural Product Communications. Roč. 10, č. 10 (2015), s. 1719-1722. ISSN 1934-578X. E-ISSN 1555-9475
R&D Projects: GA MŠMT EE2.3.30.0059; GA MŠMT ED2.1.00/03.0110; GA MŠMT LO1416
Institutional support: RVO:61388971
Keywords : Cyanobacteria * Aeruginosin-865 * Centrifugal partition chromatography
Subject RIV: EE - Microbiology, Virology
Impact factor: 0.884, year: 2015
Aeruginosin-865 was isolated from cultivated soil cyanobacteria using a combination of centrifugal partition chromatography (CPC) and gel permeation chromatography. The solubility of Aer-865 in different solvents was evaluated using the conductor-like screening model for real solvents (COSMO-RS). The CPC separation was performed in descending mode with a biphasic solvent system composed of water n-BuOH-acetic acid (5:4:1, v/v/v). The upper phase was used as a stationary phase, whereas the lower phase was employed as a mobile phase at a flow rate of 10 mL/min. The revolution speed and temperature of the separation column were 1700 rpm and 25 degrees C, respectively. Preparative CPC separation followed by gel permeation chromatography was performed on 50 mg of crude extract yielding Aer-865 (3.5 mg), with a purity over 95% as determined by HPLC. The chemical identity of the isolated compound was confirmed by comparing its spectroscopic data (UV, HRESI-MS, HRESI-MS/MS) with those of an authentic standard and data available in the literature.
Permanent Link: http://hdl.handle.net/11104/0254868
Number of the records: 1