Endogenously produced hydrogen sulfide is involved in porcine oocyte maturation in vitro

0453672 - ÚŽFG 2016 RIV NL eng J - Journal Article
Nevoral, J. - Žalmanová, T. - Zámostná, K. - Kott, T. - Kučerová-Chrpová, K. - Bodart, J. F. - Gelaude, A. - Procházka, Radek - Orsák, M. - Šulc, M. - Klein, P. - Dvořáková, M. - Weingartová, I. - Víghová, A. - Hošková, K. - Krejčová, T. - Jílek, F. - Petr, J.
Endogenously produced hydrogen sulfide is involved in porcine oocyte maturation in vitro.
Nitric Oxide-Biology and Chemistry. Roč. 51, č. 1 (2015), s. 24-35. ISSN 1089-8603. E-ISSN 1089-8611
R&D Projects: GA MZe(CZ) QJ1510138
Institutional support: RVO:67985904
Keywords : oocyte * GVBD * meiotic maturation
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.760, year: 2015

Hydrogen sulfide, one of three known gasotransmitters, is involved in physiological processes, including reproductive functions. Oocyte maturation and surrounding cumulus cell expansion play an essential role in female reproduction and subsequent embryonic development. Although the positive effects of exogenous hydrogen sulfide on maturing oocytes are well known, the role of endogenous hydrogen sulfide, which is physiologically released by enzymes, has not yet been described in oocytes. In this study, we observed the presence of Cystathionine beta-Synthase (CBS), Cystathionine gamma-Lyase (CTH) and 3-Mercaptopyruvate Sulfurtransferase (3-MPST), hydrogen sulfide-releasing enzymes, in porcine oocytes. Endogenous hydrogen sulfide production was detected in immature and matured oocytes as well as its requirement for meiotic maturation. Individual hydrogen sulfide-releasing enzymes seem to be capable of substituting for each other in hydrogen sulfide production. However, meiosis suppression by inhibition of all hydrogen sulfide-releasing enzymes is not irreversible and this effect is a result of M-Phase/Maturation Promoting Factor (MPF) and Mitogen-Activated Protein Kinase (MAPK) activity inhibition. Futhermore, cumulus expansion expressed by hyaluronic acid (HA) production is affected by the inhibition of hydrogen sulfide production. Moreover, quality changes of the expanded cumuli are indicated. These results demonstrate hydrogen sulfide involvement in oocyte maturation as well as cumulus expansion. As such, hydrogen sulfide appears to be an important cell messenger during mammalian oocyte meiosis and adequate cumulus expansion.
Permanent Link: http://hdl.handle.net/11104/0254419