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Cleavage of DNA containing 5-fluorocytosine or 5-fluorouracil by type II restriction endonucleases
- 1.0453449 - ÚOCHB 2016 RIV GB eng J - Journal Article
Olszewska, Agata - Daďová, Jitka - Mačková, Michaela - Hocek, Michal
Cleavage of DNA containing 5-fluorocytosine or 5-fluorouracil by type II restriction endonucleases.
Bioorganic & Medicinal Chemistry. Roč. 23, č. 21 (2015), s. 6885-6890. ISSN 0968-0896. E-ISSN 1464-3391
R&D Projects: GA ČR GA14-04289S
Institutional support: RVO:61388963
Keywords : modified nucleotides * DNA * restriction endonucleases * DNA polymerase * pyrimidine nucleosides
Subject RIV: CC - Organic Chemistry
Impact factor: 2.923, year: 2015
A systematic study of the cleavage of DNA sequences containing 5-fluorocytosine or 5-fluorouracil by type II restriction endonucleases (REs) was performed and the results compared with the same sequences containing natural pyrimidine bases, uracil or 5-methylcytosine. The results show that some REs recognize fluorine as a hydrogen on cytosine and cleave the corresponding sequences where the presence of m5dC leads to blocking of the cleavage. However, on uracil, the same REs recognize the F as a methyl surrogate and cleave the sequences which are not cleaved if uracil is incorporated instead of thymine. These results are interesting for understanding the recognition of DNA sequences by REs and for manipulation of the specific DNA cutting.
Permanent Link: http://hdl.handle.net/11104/0254257
Number of the records: 1