0453355 - ÚFP 2016 RIV SK eng C - Conference Paper (international conference)
Doležalová, Eva - Prukner, Václav - Lukeš, Petr - Šimek, Milan
Active but nonculturable state of Escherichia coli induced by plasma generated in gas and liquid phase.
SAPP XX: 20th Symposium on Application of Plasma Processes COST TD1208 Workshop on Application of Gaseous Plasma with Liquids Book of Contributed Papers. Bratislava: Department of Experimental Physics, Faculty of Mathematics, Physics and Informatics, Comenius University in Bratislava (Slovakia); Society for Plasma Research and Applications in cooperation with Library and Publishing Centre CU, Bratislava, Slovakia, 2015 - (Papp, P.; Országh, J.; Moravský, L.; Ribar, A.; Matejčík, Š.), s. 17-21. ISBN 978-80-8147-027-1.
[Symposium on Application of Plasma Processes COST TD1208 Workshop on Application of Gaseous Plasma with Liquids: SAPP XX /20./. Tatranská Lomnica (SK), 17.01.2015-22.01.2015]
R&D Projects: GA MŠMT(CZ) LD14080; GA MŠMT(CZ) LD13010
Grant - others:Rada Programu interní podpory projektů mezinárodní spolupráce AV ČR(CZ) M100431203; European Cooperation in Science and Technology(XE) COST TD1208
Program: M
Institutional support: RVO:61389021
Keywords : Escherichia coli * cultivation * LIVE/DEAD assay * plasma
Subject RIV: BL - Plasma and Gas Discharge Physics
Result website:
http://neon.dpp.fmph.uniba.sk/sapp/download/SAPP_XX_2015.pdf

Non-thermal atmospheric pressure plasma is extensively applied as an efficient decontamination medium; however, the decontamination is usually evaluated by the simplest classical cultivation method. We investigated the plasma induced inactivation by the conventional cultivation and fluorescent LIVE/DEAD BacLight™ Bacterial Viability techniques. The decontamination effect was studied on a culture of Escherichia coli deposited either on a solid surface or dispersed in a saline solution. We observed that the bacterial counts decreased with increasing treatment time of atmospheric pressure plasma jet and coplanar surface dielectric barrier discharge. We assume that interaction of plasma with bacteria induces a stress which does not lead to the death of bacteria, but to the loss of their ability to be cultivated, thus viable but nonculturable state (VBNC) occurs.
Permanent Link: http://hdl.handle.net/11104/0254184