Study of Transport of Biologically Important Compounds in\nEnvironment across Biological Membranes and Development of\nMethods for their Determinations

0452344 - ÚFCH JH 2016 RIV SG eng C - Conference Paper (international conference)
Navrátil, Tomáš - Šestáková, Ivana - Vodičková, H. - Jaklová Dytrtová, Jana - Gál, M.
Study of Transport of Biologically Important Compounds in
Environment across Biological Membranes and Development of
Methods for their Determinations.
Environment, Chemistry and Biology IV. Singapore: IACSIT Press, 2015, s. 43-50. International Proceedings of Chemical, Biological & Environmental Engineering, Vol. 90. ISBN 978-981-09-7382-7.
[International Conference on Environment, Chemistry and Biology (ICECB 2015) /4./. Auckland (NZ), 19.11.2015-21.11.2015]
R&D Projects: GA ČR(CZ) GAP208/12/1645
Institutional support: RVO:61388955 ; RVO:61388963
Keywords : membrane * cell * protoplast
Subject RIV: CG - Electrochemistry

Different models ofphospholipi d membranes (PLMs) have been used for better understanding of
properties and functions of real biologic al membranes. Mechanisms of transporting processes and properties
of membranes were investigated using planar (supported) PLMs. T heir electrochemical behavior was stud ied
by electrochemical impedance spectros copy (EIS). This technique was utilized to monitor fo rm ation, stability,
and transporting process across the supported PLMs (s -PLMs). The investigated synthet ic membranes were
characterized by atomic force microscopy (AFM) too. The methods fo r isolation of protoplasts from plant
cells were modified, adopted and optimized to obtain the cytoplasmic membranes containing specific
transporting systems. It has not been poss ible to fix the protoplasts in substrate pores. Therefore, the
protoplasts were destroyed and model phos pholipids (lecithin) were mixed with these parts of real protoplasts,
which contain real ion and other s pecies transporters. Further, their parameters were investigated.
Transporting processes of the environmentally important compounds and of supporting species (e .g. , heavy
metals, phytochelatin PC2, low molecular weight organ ic acids) across model and modified PLMs (in
mixture with real parts of protoplasts) were studied. The cell penetrating peptides (transportan 10 and
mastoparan X) were used to enable transfer of phytochelatin PC2. Voltammetry with hanging mercury drop
electrode (HMDE) or with solid amalga m electrodes (SAEs) was utilized for detection of transported species.
Permanent Link: http://hdl.handle.net/11104/0253338