Scanning electron microscopy and Raman spectroscopy of microorganisms related to biofuels and biopolymer production

0452285 - ÚPT 2016 DE eng C - Conference Paper (international conference)
Hrubanová, Kamila - Samek, Ota - Obruča, S. - Hároniková, A. - Krzyžánek, Vladislav
Scanning electron microscopy and Raman spectroscopy of microorganisms related to biofuels and biopolymer production.
MC 2015. Microscopy Conference Proceedings. Göttingen: DGE, 2015, s. 700-701.
[Microscopy Conference 2015. Göttingen (DE), 06.09.2015-11.09.2015]
R&D Projects: GA ČR(CZ) GA14-20012S; GA MŠMT(CZ) LO1212; GA MŠMT ED0017/01/01
Institutional support: RVO:68081731
Keywords : SEM * Raman spectroscopy
Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering

Red yeast strains such as Rhodotorula spp., Cystofilobasidium spp. and Sporobolomyces spp. are well known due to their potential applications in the field of biofuel generation and food industry. In order to utilize biomass for efficient industrial production, the optimal cultivation parameters have to be determined which in turn lead to high production of desired substances such as oil and carotenoids in the selected cell line. In this project we have studied, using scanning electron microscopy (SEM) and Raman spectroscopy techniques, how different cultivation conditions influence production of oil and carotenoids. Raman spectroscopy can be used for the determination of the oil present in the biomass and also for the determination of carotenoids as the intensity ratios of specific, selected Raman bands. SEM uses focused electron beam to gain information about morphology of cells (biomass structure) which is very important factor to study cells response on the applied stress.
Permanent Link: http://hdl.handle.net/11104/0253310