Flavonoids casticin and chrysosplenol D from Artemisia annua L. inhibit inflammation in vitro and in vivo

0451787 - ÚEM 2016 RIV US eng J - Journal Article
Li, Y.J. - Guo, Y. - Yang, Q. - Weng, X. G. - Yang, L. - Wang, Y.J. - Chen, Y. - Zhang, D. - Li, Q. - Liu, X.C. - Kan, X.X. - Chen, X. - Zhu, X.X. - Kmoníčková, E. - Zídek, Zdeněk
Flavonoids casticin and chrysosplenol D from Artemisia annua L. inhibit inflammation in vitro and in vivo.
Toxicology and Applied Pharmacology. Roč. 286, č. 3 (2015), s. 151-158. ISSN 0041-008X. E-ISSN 1096-0333
Institutional support: RVO:68378041
Keywords : Artemisia annua L. * Flavonoids * Casticin * Chrysosplenol D * Inflammation
Subject RIV: EC - Immunology
Impact factor: 3.847, year: 2015

Background: The aim of our experiments was to investigate the anti-inflammatory properties of casticin and chrysosplenol D, two flavonoids present in Artemisia annua L. /n/nMethods: Topical inflammation was induced in ICR mice using croton oil. Mice were then treated with casticin or chrysosplenol D. Cutaneous histological changes and edema were assessed. ICR mice were intragastrically administrated with casticin or chrysosplenol D followed by intraperitoneal injection of lipopolysaccharide (LPS). Mouse Raw264.7 macrophage cells were incubated with casticin or chrysosplenol D. Intracellular phosphorylation was detected, and migration was assessed by trans-well assay. HT-29/NF kappa B-luc cells were incubated with casticin or chrysosplenol D in the presence or absence of LPS, and NF-kappa B activation was quantified. /n/nResults: In mice, administration of casticin (0.5, 1 and 1.5 mu mol/cm(2)) and chrysosplenol D (1 and 1.5 mu mol/cm(2)) inhibited croton oil-induced ear edema (casticin: 29.39-64.95%; chrysosplenol D: 37.76-65.89%, all P < 0.05) in a manner similar to indomethacin (0.5, 1 and 1.5 mu mol/cm(2); 55.63-84.58%). Casticin (0.07, 0.13 and 027 mmol/kg) and chrysosplenol D (0.07, 0.14 and 0.28 mmol/kg) protected against LPS-induced systemic inflammatory response syndrome (SIRS) in mice (all P < 0.05), in a manner similar to dexamethasone (0.03 mmol/kg). Casticin and chrysosplenol D suppressed LPS-induced release of IL-1 beta, IL-6 and MCP-1, inhibited cell migration, and reduced LPS-induced I kappa B and c-JUN phosphorylation in Raw264.7 cells. JNK inhibitor SP600125 blocked the inhibitory effect of chrysosplenol Don cytokine release. /n
Permanent Link: http://hdl.handle.net/11104/0253408