Elimination of Praziquantel from waste waters both in laboratory and real scale

0451396 - ÚEB 2016 GR eng C - Conference Paper (international conference)
Maršík, Petr - Podlipná, Radka - Vaněk, Tomáš
Elimination of Praziquantel from waste waters both in laboratory and real scale.
Proceedings of the 14th International Conference on Environmental Science and Technology. Lesvos: University of the Aegean, 2015 - (Lekkas, T.). ISBN 978-960-7475-52-7. ISSN 1106-5516.
[International Conference on Environmental Science and Technology /14./. Rhodes (GR), 03.09.2015-05.09.2015]
R&D Projects: GA ČR(CZ) GA14-22593S; GA TA ČR TA01020573
Grant - others:European Regional Development Fund(XE) CZ.2.16/3.1.00/24014
Institutional support: RVO:61389030
Keywords : praziquantel * waste water * constructed wetland
Subject RIV: CE - Biochemistry
http://cest.gnest.org/cest15proceedings/public_html/papers/cest2015_00686_oral_paper.pdf

Accumulation and/or degradation of Praziquantel (PZQ) in plant organs was determined using Phragmites australis in vitro cultivated plants. The changes of PZQ content in cultivation media was monitored by HPLC analysis with PDA detection. In case of starting PZQ concentration 20 mg/l, 90 % was removed from liquid media within 21 days. Control blank samples (cultivation media without plants) showed no decrease of PZQ concentration caused by biotic degradation during the experimental period. For the proving of PZQ uptake and metabolism in plant tissues, the qualitative LC/MS/MS analysis followed with LC/MS/MS HRES of leaf extract of in vitro growing P. australis was carried out. The analysis showed, that the PZQ can be transported to the aerial organs in plants. Laboratory results were confirmed in real conditions in the constructed wetland (CW), where PZQ content was determined in the waste water applied to the system. The system offers a promising possibility to use CW for PZQ removal from agricultural as well as domestic waste-waters. The amount of PZQ in CW decreased in dependence on duration of PZQ treatment and in the direction from inflow to outflow (10 sampling points). The PZQ concentration in water reached the detection limit after sampling point 7, but the majority of the PZQ was removed after the first three sampling points.
Permanent Link: http://hdl.handle.net/11104/0252571