Raman-tweezers in microfluidic system for analysis and sorting of living cells

0451050 - ÚPT 2016 DE eng A - Abstract
Pilát, Zdeněk - Ježek, Jan - Kaňka, Jan - Zemánek, Pavel
Raman-tweezers in microfluidic system for analysis and sorting of living cells.
Invitation and Programm 116th Annual Meeting of the DGaO. Erlangen: DGaO, 2015. s. 24.
[Annual Meeting of the DGaO /116./. 26.05.2015-29.05.2015, Brno]
R&D Projects: GA MŠMT(CZ) LO1212; GA MŠMT(CZ) LD14069; GA MŠMT ED0017/01/01
Institutional support: RVO:68081731
Keywords : optical systems * spectroscopy * laser technology
Subject RIV: BH - Optics, Masers, Lasers

We built an experimental apparatus for Raman analysis and sorting of optically trapped living cells (microorganisms, cell cultures) in microfluidic environment. Optical trapping allowed non-contact spatial confinement and separation of the analyzed cells, while Raman spectroscopy was used for determination of the chemical parameters of the cells. Special microfluidic chip serves to deliver the cells to the area of Raman-tweezers and to separate and isolate the selected cells. The system allows fully automatic analysis and sorting of living microorganisms according to selected parameters of the Raman spectrum. The selected cells can be easily removed from the microfluidic chip in a form of cell-containing agarose plug. This system may find its use in various biological, biotechnological and medical disciplines.
Permanent Link: http://hdl.handle.net/11104/0252255