A deep phylogeny of viral and cellular right-hand polymerases

0450322 - BC 2016 RIV NL eng J - Journal Article
Černý, Jiří - Černá Bolfíková, B. - Zanotto, P. M. de A. - Grubhoffer, Libor - Růžek, Daniel
A deep phylogeny of viral and cellular right-hand polymerases.
Infection, Genetics and Evolution. Roč. 36, 2015-Dec (2015), s. 275-286. ISSN 1567-1348. E-ISSN 1567-7257
R&D Projects: GA ČR GAP502/11/2116; GA ČR GA15-03044S; GA ČR GAP302/12/2490; GA MŠMT(CZ) EE2.3.30.0032
EU Projects: European Commission(XE) 278976
Institutional support: RVO:60077344
Keywords : Right-hand polymerase * Polymerase evolution * Virus evolution * Structural evolution * Protein tertiary structure
Subject RIV: EE - Microbiology, Virology
Impact factor: 2.591, year: 2015

Right-hand polymerases are important players in genome replication and repair in cellular organisms as well as in viruses. All right-hand polymerases are grouped into seven related protein families: viral RNA-dependent RNA polymerases, reverse transcriptases, single-subunit RNA polymerases, and DNA polymerase families A, B, D, and Y. Although the evolutionary relationships of right-hand polymerases within each family have been proposed, evolutionary relationships between families remain elusive because their sequence similarity is too low to allow classical phylogenetic analyses. The structure of viral RNA-dependent RNA polymerases recently was shown to be useful in inferring their evolution. Here, we address evolutionary relationships between righthand polymerase families by combining sequence and structure information.We used a set of 22 viral and cellular polymerases representing all right-hand polymerase families with known protein structure. In contrast to previous studies, which focused only on the evolution of particular families, the current approach allowed us to present the first robust phylogenetic analysis unifying evolution of all right-hand polymerase families. All polymerase families branched into discrete lineages, following a fairly robust adjacency pattern.Only single-subunit RNA polymerases formed an inner group within DNA polymerase family A. RNA-dependent RNA polymerases of RNA viruses and reverse transcriptases of retroviruses formed two sister groups and were distinguishable from all other polymerases. DNA polymerases of DNA bacteriophages did not forma monophyletic group and are phylogeneticallymixed with cellular DNA polymerase families A and B.
Permanent Link: http://hdl.handle.net/11104/0254260