SEM and Raman Spectroscopy Applied to Biomass Analysis for Application in the Field of Biofuels and Food Industry

0450103 - ÚPT 2016 RIV US eng J - Journal Article
Samek, Ota - Hároniková, A. - Vaškovicová, Naděžda - Hrubanová, Kamila - Ježek, Jan - Márová, I. - Krzyžánek, Vladislav - Zemánek, Pavel
SEM and Raman Spectroscopy Applied to Biomass Analysis for Application in the Field of Biofuels and Food Industry.
Microscopy and Microanalysis. Roč. 21, S3 (2015), s. 1775-1776. ISSN 1431-9276. E-ISSN 1435-8115
R&D Projects: GA MŠMT(CZ) LO1212; GA MŠMT ED0017/01/01; GA ČR(CZ) GA14-20012S
Institutional support: RVO:68081731
Keywords : SEM * Raman spetroscopy
Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering
Impact factor: 1.730, year: 2015

A biomass of algal (Trachydiscus minutus, Botryococcus sudeticus, and Chlamydomonas sp.) and red yeast strains (Rhodotorula spp., Cystofilobasidium spp. and Sporobolomyces spp.) has been studied due to their potential applications in the field of biofuel generation and food industry. In order to utilize biomass for efficient industrial production, the optimal cultivation parameters have to be determined which in turn lead to high production of desired substances such as oil and carotenoids in the selected cell line [2]. Main aim of our investigations was to study – using scanning electron microscopy (SEM) and Raman spectroscopy techniques – how different cultivation conditions influence production of oil and carotenoids. Raman spectroscopy can be used for the determination of the oil present in the biomass and also for the determination of carotenoids as the intensity ratios of specific, selected Raman bands. SEM uses electron beams to gain information about morphology of cells (biomass structure) which is very important factor to study cells response on the applied stress. In our experiments we have observed different morphology when employing SEM to study cells of Cystofilobasidium capitatum cultivating in the two media with different carbon to nitrogen (C/N) ratio. C/N ratio of cultivation media has an influence to the production of yeast lipids or carotenoids. First growth medium (medium 1) indicates positive results to carotenoid production and second medium (medium 2) with high C/N ratio leads to an increased lipid production which can be indicated using Raman spectroscopy.
Permanent Link: http://hdl.handle.net/11104/0251508