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Synthesis and analysis of quantum dot conjugates intended for fret sensor
- 1.0449636 - ÚIACH 2016 RIV CZ eng C - Conference Paper (international conference)
Datinská, Vladimíra - Klepárník, Karel - Belšánová, Barbora - Minárik, M. - Foret, František
Synthesis and analysis of quantum dot conjugates intended for fret sensor.
CECE 2015. 12th International Interdisciplinary Meeting on Bioanalysis. Brno: Ústav analytické chemie AV ČR, v. v. i, 2015 - (Foret, F.; Křenková, J.; Drobníková, I.; Klepárník, K.), s. 110-112. ISBN 978-80-904959-3-7.
[CECE 2015. International Interdisciplinary Meeting on Bioanalysis /12./. Brno (CZ), 21.09.2015-23.09.2015]
R&D Projects: GA TA ČR(CZ) TA02010672; GA ČR(CZ) GA14-28254S
Institutional support: RVO:68081715
Keywords : quantum dots * Fӧrster resonance energy transfer * DNA mutation detection
Subject RIV: CB - Analytical Chemistry, Separation
http://www.ce-ce.org/CECE2015/CECE%202015%20proceedings_full.pdf
Our work is focused on synthesis of sensor based on Fӧrster resonance energy transfer (FRET), which is aimed to be used for DNA mutation detection. The sensor is based on the attachment of laboratory synthesized quantum dot (QD) to ssDNA. A complementary chain of a sample is labeled by a fluorescent dye. Thus, only if dsDNA between both complementary chains is formed, the energy from QD (donor) can be transferr ed to the dye acceptor) and FRET is observed.
Permanent Link: http://hdl.handle.net/11104/0251151
Number of the records: 1