SDS-PAGE and gel IEF – tool for differentiation of methicillin-resistant and methicillin-sensitive strains of Staphylococcus aureus

0449521 - ÚIACH 2016 RIV US eng J - Journal Article
Tesařová, Marie - Horká, Marie - Moravcová, Dana - Svojanovská, Lenka - Mlynariková, K. - Růžička, F.
SDS-PAGE and gel IEF – tool for differentiation of methicillin-resistant and methicillin-sensitive strains of Staphylococcus aureus.
Current Microbiology. Roč. 72, č. 3 (2016), s. 315-320. ISSN 0343-8651. E-ISSN 1432-0991
R&D Projects: GA MV VG20112015021
Institutional support: RVO:68081715
Keywords : methicillin-resistant and methicillin-sensitive Staphylococcus aureus * sodium dodecylsulphate polyacrylamide gel electrophoresis * gel isoelectric focusing * precipitated proteins
OECD category: Analytical chemistry
Impact factor: 1.322, year: 2016

The methicillin-resistant Staphylococcus aureus causes difficult-to-treat healthcare-associated infections in humans. For fast and effective selection of an appropriate antibiotic therapy, it is essential to have rapid and reliable methods for differentiation of methicillin-resistant Staphylococcus aureus from less dangerous methicillin-sensitive Staphylococcus aureus. There have been many methods for the identification of methicillin-resistant Staphylococcus aureus described but none has been accepted as an international standard. The most commonly used techniques such as phenotyping and genotyping have a few disadvantages, for instance, these techniques are not reproducible and stable. In addition, they are time-consuming, expensive, and they are not capable to distinguish all Staphylococcus aureus strains. In this study, the methicillin-resistant and methicillin-sensitive Staphylococcus aureus isolates obtained from patients were extracted in hot water. The released proteins were characterized by sodium dodecylsulphate polyacrylamide gel electrophoresis and gel isoelectric focusing. These two methods were able to differentiate among tested bacterial strains. The proposed methods are time saving, they are applicable in standard biochemical laboratories, and they do not require any expensive equipment.
Permanent Link: http://hdl.handle.net/11104/0251069