Effect of various concentrations of Ti in hydrocarbon plasma polymer films on the adhesion, proliferation and differentiation of human osteoblast-like MG-63 cells

0449222 - FGÚ 2016 RIV NL eng J - Journal Article
Vandrovcová, Marta - Grinevich, A. - Drábik, M. - Kylián, O. - Hanuš, J. - Staňková, Ľubica - Lisá, Věra - Choukourov, A. - Slavínská, D. - Biederman, H. - Bačáková, Lucie
Effect of various concentrations of Ti in hydrocarbon plasma polymer films on the adhesion, proliferation and differentiation of human osteoblast-like MG-63 cells.
Applied Surface Science. Roč. 357, part A (2015), s. 459-472. ISSN 0169-4332. E-ISSN 1873-5584
R&D Projects: GA ČR(CZ) GAP108/12/1168; GA ČR(CZ) GA14-04790S
Institutional support: RVO:67985823
Keywords : metal carbon composite films * surface wettability * nanoscale roughness * osteoblasts * bone implants
Subject RIV: EI - Biotechnology ; Bionics
Impact factor: 3.150, year: 2015

The human osteoblast-like MG-63 cells cultured on pure ppCH films and on films with 20 at% of Ti showed relatively high concentrations of ICAM-1, a marker of cell immune activation. Lower concentrations of Ti (mainly 5 at%) improved cell adhesion and osteogenic differentiation, as revealed by higher concentrations of talin, vinculin and osteocalcin. Higher Ti concentrations (15 at%) supported cell growth, as indicated by the highest final cell population densities on day 7 after seeding. Thus, enrichment of ppCH films with appropriate concentrations of Ti makes these films more suitable for potential coating of bone implants
Permanent Link: http://hdl.handle.net/11104/0250807