Fluorescent magnetic nanoparticles for cell labeling: flux synthesis of manganite particles and novel functionalization of silica shell

0448628 - FZÚ 2016 RIV US eng J - Journal Article
Kačenka, Michal - Kaman, Ondřej - Kikerlová, S. - Pavlů, B. - Jirák, Zdeněk - Jirák, D. - Herynek, Vít - Černý, J. - Chaput, F. - Laurent, S. - Lukeš, I.
Fluorescent magnetic nanoparticles for cell labeling: flux synthesis of manganite particles and novel functionalization of silica shell.
Journal of Colloid and Interface Science. Roč. 47, Jun (2015), s. 97-106. ISSN 0021-9797. E-ISSN 1095-7103
R&D Projects: GA ČR(CZ) GAP108/11/0807; GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:68378271 ; RVO:68378041
Keywords : manganites * magnetic nanoparticles * molten salt synthesis * silica coating * dual probes * MRI * cell labeling
Subject RIV: BM - Solid Matter Physics ; Magnetism
Impact factor: 3.782, year: 2015

Novel synthetic approaches for the development of multimodal imaging agents are demonstrated. The magnetic cores are based on La0.63Sr0.37MnO3 manganite prepared by molten salt synthesis followed by thermal treatment in protective silica shells. The cores are then isolated and covered de novo with a hybrid silica layer which incorporates fluorescent probe synthesized from rhodamine, piperazine spacer, and 3-iodopropyltrimethoxysilane. The aminoalkyltrialkoxysilanes are strictly avoided and the resulting particles are hydrolytically stable. Structural and magnetic studies confirm the importance of the thermal treatment and demonstrate that no mechanical processing is required. Cell viability tests show negligible at concentrations whereby excellent labeling is achieved. Predominant localization of nanoparticles in lysosomes is confirmed by immunofluorescence staining. Relaxometric and biological studies suggest that the functionalized nanoparticles are suitable for imaging applications.
Permanent Link: http://hdl.handle.net/11104/0250275