N-(2-hydroxypropyl) methacrylamide based cryogels - synthesis and biomimetic modification for stem cell applications

0448561 - ÚMCH 2016 RIV CZ eng J - Journal Article
Golunova, Anna - Jaroš, J. - Jurtíková, V. - Kotelnikov, Ilya - Kotek, Jiří - Hlídková, Helena - Streit, L. - Hampl, A. - Rypáček, František - Proks, Vladimír
N-(2-hydroxypropyl) methacrylamide based cryogels - synthesis and biomimetic modification for stem cell applications.
Physiological Research. Roč. 64, Suppl. 1 (2015), S19-S27. ISSN 0862-8408. E-ISSN 1802-9973
R&D Projects: GA ČR(CZ) GA13-29009S; GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:61389013
Keywords : hydrogels * cryogels * stem cells
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 1.643, year: 2015
http://www.biomed.cas.cz/physiolres/pdf/64%20Suppl%201/64_S19.pdf

The design of favorable mechanical properties and suitable surface modifications of hydrogels in order to stimulate specific cell response is a great challenge. N-(2-Hydroxypropyl) methacryl-amide (HPMA) was utilized to form macroporous cryogel scaffolds for stem cell applications. Furthermore, one group of scaffolds was enhanced by copolymerization of HPMA with methacryloyl-GGGRGDS-OH peptide in an effort to integrate biomimetic adhesion sites. The cryogels were characterized by stiffness and equilibrium swelling measurements as well as by scanning electron microscopy. Cell culture experiments were performed with human adipose-derived stem cells and substrates were found completely non-toxic. Moreover, RGDS-enriched cryogels supported cell attachment, spreading and proliferation, so they can be considered suitable for designed aims.
Permanent Link: http://hdl.handle.net/11104/0253907