Dose-dependent micronuclei formation in normal human fibroblasts exposed to proton radiation

0448253 - ÚJF 2016 RIV US eng J - Journal Article
Litvinchuk, Alexandra - Vachelová, Jana - Jelínek Michaelidesová, Anna - Wagner, Richard - Davídková, Marie
Dose-dependent micronuclei formation in normal human fibroblasts exposed to proton radiation.
Radiation and Environmental Biophysics. Roč. 54, č. 3 (2015), s. 327-334. ISSN 0301-634X. E-ISSN 1432-2099
R&D Projects: GA ČR(CZ) GBP108/12/G108; GA MŠMT LM2011019
Institutional support: RVO:61389005
Keywords : human fibroblasts * proton radiation * micronuclei assay * biodosimetry
Subject RIV: BO - Biophysics
Impact factor: 1.923, year: 2015

Micronuclei are small extranuclear bodies resulting from chromosome fragments or the whole chromosomes secluded from daughter nuclei during mitosis. The number of radiation-induced micronuclei reflects the level of chromosomal damage and relates to an absorbed dose and quality of incident ionizing radiation. The aim of the present study was to determine the micronucleus formation as a specific biological marker for acute radiation-induced DNA damage in normal human fibroblasts exposed to 30-MeV protons and Co-60 gamma radiation. We found a linear increase in binuclear cells containing micronuclei for absorbed doses from 1 to 5 Gy for both radiation modalities. However, the total number of micronuclei in binuclear cells follows a linear-quadratic dose dependence. In case of human exposure to mixed radiation fields or high LET radiation, the proportion of binuclear cells containing micronuclei from all binuclear cells can thus serve as a good biomarker of radiation-induced DNA damage.
Permanent Link: http://hdl.handle.net/11104/0250018