Nippotaenia mogurndae Yamaguti et Myiata, 1940 (Cestoda, Nippotaeniidea): first data on spermiogenesis and sperm ultrastructure

0447678 - BC 2016 RIV DE eng J - Journal Article
Bruňanská, M. - Bílý, Tomáš - Nebesářová, Jana
Nippotaenia mogurndae Yamaguti et Myiata, 1940 (Cestoda, Nippotaeniidea): first data on spermiogenesis and sperm ultrastructure.
Parasitology Research. Roč. 114, č. 4 (2015), s. 1443-1453. ISSN 0932-0113. E-ISSN 1432-1955
R&D Projects: GA TA ČR(CZ) TE01020118
Institutional support: RVO:60077344
Keywords : Nippotaeniidea * Nippotaeniamogurndae * spermiogenesis * spermatozoon * ultrastructure
Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering
Impact factor: 2.027, year: 2015

Spermiogenesis and the spermatozoon ultrastructure of the cestode Nippotaenia mogurndae Yamaguti et Myiata, 1940 (Nippotaeniidea), a parasite of Perccottus glenii Dubowski, 1877 (Perciformes: Odontobutidae), have been investigated by means of transmission electron microscopy, cytochemical staining with periodic acid-thiosemicarbazide-silver proteinate (PA-TSC-SP) for glycogen, and electron tomography. The process of spermatozoon formation is characterised by the presence of (1) two centrioles without typical striated rootlets, (2) a single intercentriolar body, (3) a flagellar rotation (free flagellum plus flagellar bud), and (4) a complete proximodistal fusion. The mature spermatozoon of N. mogurndae contains a single helicoidal crested body, one axoneme of the 9+"1" trepaxonematan structure, parallel cortical microtubules arranged in a ring in the anterior region of the cell, and a spiraled nucleus encircling the axoneme. Intracellular components are situated in a moderately electron-dense cytoplasm, containing glycogen in the principal regions (II, III, IV) of the spermatozoon. Application of electron tomography has revealed a helicoidal nature of the central electron-dense core in the central cylinder of the axoneme in parasitic cestodes for the first time. The patterns of spermiogenesis and spermatozoon ultrastructure resemble most closely those in mesocestoidids and may reflect the relationships between Nippotaeniidea and Mesocestoididae.
Permanent Link: http://hdl.handle.net/11104/0249447