0447676 - BC 2016 RIV GB eng J - Journal Article
Brabec, Jan - Kostadinova, Aneta - Scholz, Tomáš - Littlewood, D. T. J.
Complete mitochondrial genomes and nuclear ribosomal RNA operons of two species of Diplostomum (Platyhelminthes: Trematoda): a molecular resource for taxonomy and molecular epidemiology of important fish pathogens.
Parasites & Vectors. Roč. 8, JUN 19 2015 (2015), s. 336. ISSN 1756-3305. E-ISSN 1756-3305
R&D Projects: GA MŠMT(CZ) EE2.3.30.0032; GA ČR(CZ) GA15-14198S
Grant - others:GA MŠk(CZ) LM2010005
Institutional support: RVO:60077344
Keywords : Diplostomum (Platyhelminthes: Trematoda) * fish pathogens * mitochondrial genome * ribosomal RNA * illumina next-generation sequencing * phylogeny
Subject RIV: EG - Zoology
Impact factor: 3.234, year: 2015
DOI: https://doi.org/10.1186/s13071-015-0949-4

Results: We characterised novel complete mt genomes and nuclear rRNA operons of two closely related species, Diplostomum spathaceum and D. pseudospathaceum. Comparative mt genome assessment revealed that the cox1 gene and its 'barcode' region used for molecular identification are the most conserved regions; instead, nad4 and nad5 genes were identified as most promising molecular diagnostic markers. Using the novel data, we provide the first genome wide estimation of the phylogenetic relationships of the order Diplostomida, one of the two fundamental lineages of the Digenea. Analyses of the mitogenomic data invariably recovered the Diplostomidae as a sister lineage of the order Plagiorchiida rather than as a basal lineage of the Diplostomida as inferred in rDNA phylogenies; this was concordant with the mt gene order of Diplostomum spp. exhibiting closer match to the conserved gene order of the Plagiorchiida. Conclusions: Complete sequences of the mt genome and rRNA operon of two species of Diplostomum provide a valuable resource for novel genetic markers for species delineation and large-scale molecular epidemiology and disease ecology studies based on the most accessible life-cycle stages of eye flukes.

Permanent Link: http://hdl.handle.net/11104/0249445