Cloacal microbiome structure in a long-distance migratory bird assessed using deep 16sRNA pyrosequencing

0447548 - ÚBO 2016 RIV US eng J - Journal Article
Kreisinger, Jakub - Čížková, Dagmar - Kropáčková, L. - Albrecht, Tomáš
Cloacal microbiome structure in a long-distance migratory bird assessed using deep 16sRNA pyrosequencing.
PLoS ONE. Roč. 10, č. 9 (2015), e0137401. ISSN 1932-6203. E-ISSN 1932-6203
R&D Projects: GA ČR(CZ) GAP506/12/2472; GA MŠMT EE2.3.20.0303
Institutional support: RVO:68081766
Keywords : gastrointestinal microbiota * gut microbiome * sexual selection * immune-system * barn swallows * bacteria * evolution * communities * diversity * taxonomy
Subject RIV: EG - Zoology
Impact factor: 3.057, year: 2015

Effects of vertebrate-associated microbiota on physiology and health are of significant interest in current biological research. Most previous studies have focused on host-microbiota interactions in captive-bred mammalian models. These interactions and their outcomes are still relatively understudied, however, in wild populations and non-mammalian taxa. Using deep pyrosequencing, we described the cloacal microbiome (CM) composition in free living barn swallows Hirundo rustica, a long-distance migratory passerine bird. Barn swallow CM was dominated by bacteria of the Actinobacteria, Proteobacteria and Firmicutes phyla. Bacteroidetes, which represent an important proportion of the digestive tract microbiome in many vertebrate species, was relatively rare in barn swallow CM (< 5%). CM composition did not differ between males and females. A significant correlation of CM within breeding pair members is consistent with the hypothesis that cloacal contact during within-pair copulation may promote transfer of bacterial assemblages. This effect on CM composition had a relatively low effect size, however, possibly due to the species’ high level of sexual promiscuity.
Permanent Link: http://hdl.handle.net/11104/0249357