A simple microviscometric approach based on Brownian motion tracking

0446344 - BFÚ 2016 RIV US eng J - Journal Article
Hnyluchová, Z. - Bjalončikova, P. - Karas, P. - Mravec, F. - Halasová, T. - Pekar, M. - Kubala, Lukáš - Víteček, Jan
A simple microviscometric approach based on Brownian motion tracking.
Review of Scientific Instruments. Roč. 86, č. 2 (2015). ISSN 0034-6748. E-ISSN 1089-7623
R&D Projects: GA ČR(CZ) GCP305/12/J038
Grant - others:GA MŠk(CZ) ED1.100/02/0123
Institutional support: RVO:68081707
Keywords : MULTIPLE-PARTICLE TRACKING * MICRORHEOLOGY * IMAGE
Subject RIV: BO - Biophysics
Impact factor: 1.336, year: 2015

Viscosity-an integral property of a liquid-is traditionally determined by mechanical instruments. The most pronounced disadvantage of such an approach is the requirement of a large sample volume, which poses a serious obstacle, particularly in biology and biophysics when working with limited samples. Scaling down the required volume by means of microviscometry based on tracking the Brownian motion of particles can provide a reasonable alternative. In this paper, we report a simple microviscometric approach which can be conducted with common laboratory equipment. The core of this approach consists in a freely available standalone script to process particle trajectory data based on a Newtonian model. In our study, this setup allowed the sample to be scaled down to 10 mu l. The utility of the approach was demonstrated using model solutions of glycerine, hyaluronate, and mouse blood plasma. Therefore, this microviscometric approach based on a newly developed freely available script can be suggested for determination of the viscosity of small biological samples (e.g., body fluids). (C) 2015 AIP Publishing LLC.
Permanent Link: http://hdl.handle.net/11104/0248359