Lysine, Arginine, and Histidine Residues in Peptide-Catalyzed Hydrogen Evolution at Mercury Electrodes

0446306 - BFÚ 2016 RIV DE eng J - Journal Article
Dorčák, Vlastimil - Vargová, Veronika - Ostatná, Veronika - Paleček, Emil
Lysine, Arginine, and Histidine Residues in Peptide-Catalyzed Hydrogen Evolution at Mercury Electrodes.
Electroanalysis. Roč. 27, č. 4 (2015), s. 910-916. ISSN 1040-0397. E-ISSN 1521-4109
R&D Projects: GA ČR(CZ) GA15-15479S; GA ČR(CZ) GA13-00956S
Institutional support: RVO:68081707
Keywords : AMALGAM ELECTRODES * STRIPPING CHRONOPOTENTIOMETRY * CARBON ELECTRODES
Subject RIV: BO - Biophysics
Impact factor: 2.471, year: 2015

Involvement of hexapeptides composed of lysine (Lys(6)), arginine (Arg(6)) or histidine (His(6)) in the catalytic hydrogen evolution reaction (CHER) was studied. Using McIlvaine buffer, we changed the concentration of citric acid (proton donor in the CHER) either (a) by changing the buffer concentration at constant pH or (b) by changing the pH at constant buffer concentration. These two approaches showed almost the same catalytic behavior of Lys(6) and Arg(6) but strikingly different ones in the case of His(6). The results suggested that at neutral pH His residue is much weaker catalyzer than those of Lys or Arg.
Permanent Link: http://hdl.handle.net/11104/0248302