Phospholipase D affects translocation of NPR1 to the nucleus in Arabidopsis thaliana

0446190 - ÚEB 2016 RIV CH eng J - Journal Article
Janda, Martin - Šašek, Vladimír - Chmelařová, H. - Andrejch, J. - Nováková, Miroslava - Hajšlová, J. - Burketová, Lenka - Valentová, O.
Phospholipase D affects translocation of NPR1 to the nucleus in Arabidopsis thaliana.
Frontiers in Plant Science. Roč. 6, FEB 18 (2015). ISSN 1664-462X. E-ISSN 1664-462X
R&D Projects: GA ČR(CZ) GAP501/11/1654
Institutional support: RVO:61389030
Keywords : n-butanol * NPR1 * salicylic acid
Subject RIV: CE - Biochemistry
Impact factor: 4.495, year: 2015

Phytohormone salicylic acid (SA) is a crucial component of plant-induced defense against biotrophic pathogens. Although the key players of the SA pathway are known, there are still gaps in the understanding of the molecular mechanism and the regulation of particular steps. In our previous research, we showed in Arabidopsis suspension cells that n-butanol, which specifically modulates phospholipase D activity, significantly suppresses the transcription of the pathogenesis related (PR-1) gene, which is generally accepted as the SA pathway marker. In the presented study, we have investigated the site of n-butanol action in the SA pathway. We were able to show in Arabidopsis plants treated with SA that n-butanol inhibits the transcription of defense genes (PR-1, WRKY38). Fluorescence microscopy of Arabidopsis thaliana mutants expressing 35S::NPR1-GFP (nonexpressor pathogenesis related 1) revealed significantly decreased nuclear localization of NPR1 in the presence of n-butanol. On the other hand, n-butanol did not decrease the nuclear localization of NPR1 in 35S::npr1C82A-GFP and 35S::npr1C216A-GFP mutants constitutively expressing NPR1 monomers. Mass spectrometric analysis of plant extracts showed that n-butanol significantly changes the metabolic fingerprinting while t-butanol had no effect. We found groups of the plant metabolites, influenced differently by SA and n-butanol treatment. Thus, we proposed several metabolites as markers for n-butanol action.
Permanent Link: http://hdl.handle.net/11104/0248175