alpha-L-Rhamnosyl-beta-D-glucosidase (Rutinosidase) from Aspergillus niger: Characterization and Synthetic Potential of a Novel Diglycosidase

0444488 - MBÚ 2016 RIV DE eng J - Journal Article
Šimčíková, Daniela - Kotík, Michael - Weignerová, Lenka - Halada, Petr - Pelantová, Helena - Adamcová, K. - Křen, Vladimír
alpha-L-Rhamnosyl-beta-D-glucosidase (Rutinosidase) from Aspergillus niger: Characterization and Synthetic Potential of a Novel Diglycosidase.
Advanced Synthesis & Catalysis. Roč. 357, č. 1 (2015), s. 107-117. ISSN 1615-4150. E-ISSN 1615-4169
R&D Projects: GA MŠMT(CZ) LD13042; GA MŠMT(CZ) 7E11011
Institutional support: RVO:61388971
Keywords : diglycosidase * enzyme catalysis * glycosides
Subject RIV: CC - Organic Chemistry
Impact factor: 6.453, year: 2015

We report the first heterologous production of a fungal rutinosidase (6-O-alpha-L-rhamnopyranosyl-beta-d-glucopyranosidase) in Pichia pastoris. The recombinant rutinosidase was purified from the culture medium to apparent homogeneity and biochemically characterized. The enzyme reacts with rutin and cleaves the glycosidic linkage between the disaccharide rutinose and the aglycone. Furthermore, it exhibits high transglycosylation activity, transferring rutinose from rutin as a glycosyl donor onto various alcohols and phenols. The utility of the recombinant rutinosidase was demonstrated by its use for the synthesis of a broad spectrum of rutinosides of primary (saturated and unsaturated), secondary, acyclic and phenolic alcohols as well as for the preparation of free rutinose. Moreover, the alpha-L-rhamnosidase-catalyzed synthesis of a chromogenic substrate for a rutinosidase assay - para-nitrophenyl beta-rutinoside - is described.
Permanent Link: http://hdl.handle.net/11104/0247003