Development and characterization of microsatellite markers from the humivorous termite Cavitermes tuberosus (Isoptera: Termitinae) using pyrosequencing technology

0444428 - ÚOCHB 2016 RIV NL eng J - Journal Article
Fournier, D. - Hanus, Robert - Roisin, Y.
Development and characterization of microsatellite markers from the humivorous termite Cavitermes tuberosus (Isoptera: Termitinae) using pyrosequencing technology.
Conservation genetics resources. Roč. 7, č. 2 (2015), s. 521-524. ISSN 1877-7252. E-ISSN 1877-7260
Institutional support: RVO:61388963
Keywords : Cavitermes tuberosus * termite * microsatellite * pyrosequencing * population genetics
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 0.446, year: 2015

Eighteen microsatellite markers from the humivorous termite Cavitermes tuberosus (Isoptera: Termitidae: Termitinae) were developed using a procedure of microsatellite-enriched libraries pyrosequencing. They were optimized in four multiplex PCR sets, and tested on 38 individuals collected in French Guiana. The number of alleles per locus ranged from 3 to 13. The expected and observed heterozygosities varied from 0.279 to 0.867 and from 0.237 to 0.789, respectively. Cross-species amplifications of these loci were performed in eight other neotropical species of Termitidae. These new microsatellite markers will constitute useful tools to study population genetics, reproductive strategies and dispersal patterns of C. tuberosus, which is a common representative of the humivorous guild, fulfilling an essential function in soil nutrient recycling.
Permanent Link: http://hdl.handle.net/11104/0247105