Membrane damage and active but nonculturable state in liquid cultures of Escherichia coli treated with an atmospheric pressure plasma jet

0444287 - ÚFP 2016 RIV CH eng J - Journal Article
Doležalová, Eva - Lukeš, Petr
Membrane damage and active but nonculturable state in liquid cultures of Escherichia coli treated with an atmospheric pressure plasma jet.
Bioelectrochemistry. Roč. 103, June (2015), s. 7-14. ISSN 1567-5394. E-ISSN 1878-562X
R&D Projects: GA MŠMT(CZ) LD14080
Grant - others:Rada Programu interní podpory projektů mezinárodní spolupráce AV ČR(CZ) M100431203
Program: M
Institutional support: RVO:61389021
Keywords : Electrical discharge * Escherichia coli * LIVE/DEAD assay * Viability * culturability * Lipid peroxidation
Subject RIV: EE - Microbiology, Virology
Impact factor: 3.556, year: 2015
http://dx.doi.org/10.1016/j.bioelechem.2014.08.018

Electrical discharge plasmas can efficiently inactivate various microorganisms. Inactivation mechanisms caused by plasma, however, are not fully understood because of the complexity of both the plasma and biological systems.We investigated plasma-induced inactivation of Escherichia coli in water and mechanisms by which plasma affects bacterial cell membrane integrity.Atmospheric pressure argon plasma jet generated at ambient air in direct contact with bacterial suspension was used as a plasma source.We determined significantly lower counts of E. coli after treatment by plasma when they were assayed using a conventional cultivation technique than using a fluorescence-based LIVE/DEAD staining method,which indicated that bacteria may have entered the viable-but-nonculturable state (VBNC).We did not achieve resuscitation of these non-culturable cells, however,we detected their metabolic activity through the analysis of cellular mRNA,which suggests that cells may have been rather in the
Permanent Link: http://hdl.handle.net/11104/0246838