Microchip Capillary Electrophoresis Protocols

0444012 - ÚIACH 2016 RIV US eng M - Monography Chapter
Szekrényes, A. - Partyka, Jan - Varadi, C. - Křenková, Jana - Foret, František - Guttman, András
Sample Preparation for N-Glycosylation Analysis of Therapeutic Monoclonal Antibodies by Electrophoresis.
Microchip Capillary Electrophoresis Protocols. vol. 1274. New York: Springer Science+Business Media, 2015, s. 183-195. Methods in Molecular Biology. ISBN 978-1-4939-2352-6
R&D Projects: GA ČR(CZ) GAP301/11/2055
Institutional support: RVO:68081715
Keywords : capillary electrophoresis * monoclonal antibodies * sample preparation
Subject RIV: CB - Analytical Chemistry, Separation
https://link.springer.com/protocol/10.1007%2F978-1-4939-2353-3_16

We describe the protocols to characterize therapeutic monclonal antibodies involving affinity isolation, enzymatic release, labeling, purification and separation of N-glycans by CE/LIF.
Permanent Link: http://hdl.handle.net/11104/0246618