Polymerase synthesis of DNA labelled with benzylidene cyanoacetamide-based fluorescent molecular rotors: fluorescent light-up probes for DNA-binding proteins

0443739 - ÚOCHB 2016 RIV GB eng J - Journal Article
Dziuba, Dmytro - Pohl, Radek - Hocek, Michal
Polymerase synthesis of DNA labelled with benzylidene cyanoacetamide-based fluorescent molecular rotors: fluorescent light-up probes for DNA-binding proteins.
Chemical Communications. Roč. 51, č. 23 (2015), s. 4880-4882. ISSN 1359-7345. E-ISSN 1364-548X
R&D Projects: GA ČR GBP206/12/G151
Institutional support: RVO:61388963
Keywords : enzyme amplification reaction * modified oligonucleotides * nucleoside triphosphates
Subject RIV: CC - Organic Chemistry
Impact factor: 6.567, year: 2015
http://pubs.rsc.org/en/content/articlepdf/2015/cc/c5cc00530b

Viscosity-sensitive fluorophores, fluorescent molecular rotors based on aminobenzylidene-cyanoacetamide moiety, were tethered to 2'-deoxycytidine triphosphate via a propargylamine linker and incorporated into DNA by polymerases in primer extension, nicking enzyme amplification or PCR. DNA probes incorporating modified nucleosides show a light-up response upon binding to a protein.
Permanent Link: http://hdl.handle.net/11104/0246447