Magnetically responsive enzyme powders

0443677 - ÚVGZ 2016 RIV NL eng J - Journal Article
Pospišková, K. - Šafařík, Ivo
Magnetically responsive enzyme powders.
Journal of Magnetism and Magnetic Materials. Roč. 380, APR 2015 (2015), s. 197-200. ISSN 0304-8853. E-ISSN 1873-4766
R&D Projects: GA MŠMT(CZ) LD13021
Institutional support: RVO:67179843
Keywords : enzyme powders * cross-linking * magnetic modification * magnetic separation * magnetic iron oxides particles * microwave-assisted synthesis
Subject RIV: CE - Biochemistry
Impact factor: 2.357, year: 2015

Powdered enzymes were transformed into their insoluble magnetic derivatives retaining their catalytic activity. Enzyme powders (e.g., trypsin and lipase) were suspended in various liquid media not allowing their solubilization (e.g., saturated ammonium sulfate and highly concentrated polyethylene glycol solutions, ethanol, methanol, 2-propanol) and subsequently cross-linked with glutaraldehyde. Magnetic modification was successfully performed at low temperature in a freezer (−20 °C) using magnetic iron oxides nano- and microparticles prepared by microwave-assisted synthesis from ferrous sulfate. Magnetized cross-linked enzyme powders were stable at least for two months in water suspension without leakage of fixed magnetic particles. Operational stability of magnetically responsive enzymes during eight repeated reaction cycles was generally without loss of enzyme activity. Separation of magnetically modified cross-linked powdered enzymes from reaction mixtures was significantly simplified due to their magnetic properties.
Permanent Link: http://hdl.handle.net/11104/0246390