Cholesterol under oxidative stress — How lipid membranes sense oxidation as cholesterol is being replaced by oxysterols

0443291 - ÚFCH JH 2016 RIV US eng J - Journal Article
Kulig, W. - Olžyńska, Agnieszka - Jurkiewicz, Piotr - Kantola, A. M. - Komulainen, S. - Manna, M. - Pourmousa, M. - Vazdar, M. - Cwiklik, Lukasz - Rog, T. - Khelashvili, G. - Harries, D. - Telkki, V. - V. - Hof, Martin - Vattulainen, I. - Jungwirth, Pavel
Cholesterol under oxidative stress — How lipid membranes sense oxidation as cholesterol is being replaced by oxysterols.
Free Radical Biology and Medicine. Roč. 84, JUL 2015 (2015), s. 30-41. ISSN 0891-5849. E-ISSN 1873-4596
R&D Projects: GA ČR GBP208/12/G016
Institutional support: RVO:61388955 ; RVO:61388963
Keywords : phospholipid bilayers * oxysterols * molecular dynamics simulations
Subject RIV: CF - Physical ; Theoretical Chemistry
Impact factor: 5.784, year: 2015

The behavior of oxysterols in phospholipid membranes and their effects on membrane properties were investigated by means of dynamic light scattering, fluorescence spectroscopy, NMR, and extensive atomistic simulations. Two families of oxysterols were scrutinized — tail-oxidized sterols, which are mostly produced by enzymatic processes, and ring-oxidized sterols, formed mostly via reactions with free radicals. The former family of sterols was found to behave similar to cholesterol in terms of molecular orientation, roughly parallel to the bilayer normal, leading to increasing membrane stiffness and suppression of its membrane permeability. In contrast, ring-oxidized sterols behave quantitatively differently from cholesterol. They acquire tilted orientations and therefore disrupt the bilayer structure with potential implications for signaling and other biochemical processes in the membranes.
Permanent Link: http://hdl.handle.net/11104/0246022