Tumor Progression Is Associated with Increasing CD11b(+) Cells and CCL2 in Lewis Rat Sarcoma

0443255 - ÚŽFG 2016 RIV GR eng J - Journal Article
Mishra, Rajbardhan - Kovalská, Jana - Janda, Jozef - Vannucci, Luca - Rajmon, R. - Horák, Vratislav
Tumor Progression Is Associated with Increasing CD11b(+) Cells and CCL2 in Lewis Rat Sarcoma.
Anticancer Research. Roč. 35, č. 2 (2015), s. 703-712. ISSN 0250-7005. E-ISSN 1791-7530
R&D Projects: GA MŠMT ED2.1.00/03.0124
Institutional support: RVO:67985904 ; RVO:61388971
Keywords : Lewis rat sarcoma * CD11b+cells * neutrophils
Subject RIV: FD - Oncology ; Hematology
Impact factor: 1.895, year: 2015

Tumor models are essential for basic anticancer research and development of novel therapies. In this study, we used a rat sarcoma model in which subcutaneous tumor develops after D6 cell inoculation. The aim of the current study was to analyze changes in haematological parameters, immune cell sub-populations and cytokine profiling during tumor growth, after tumor excision and after second inoculation of D6 cells. Tumor progression was found to be associated with an increased number of leukocytes and increased proportion of CD11b(+) cells in peripheral blood. Serum concentration of chemokine (c-c motif) ligand 2, L-selectin and intra cellular adhesion molecule-1 also increased with growing tumor. However, the proportion of CD4+, CD8+ and MHC II+ cells decreased with growth of tumors. After tumor excision, all these parameters returned to pre-inoculation levels and did not change even after a second inoculation of D6 cells. Moreover, absence of secondary tumors after second inoculation of D6 cells gives an insight into development of antitumor immunity stimula ted by primary tumor.
Permanent Link: http://hdl.handle.net/11104/0245993