Toward structured macroporous hydrogel composites: electron beam-initiated polymerization of layered cryogels

0443231 - ÚMCH 2016 RIV US eng J - Journal Article
Golunova, Anna - Chvátil, David - Krist, Pavel - Jaroš, J. - Jurtíková, V. - Pospíšil, J. - Kotelnikov, Ilya - Abelová, Lucie - Kotek, Jiří - Sedlačík, Tomáš - Kučka, Jan - Koubková, Jana - Studenovská, Hana - Streit, L. - Hampl, A. - Rypáček, František - Proks, Vladimír
Toward structured macroporous hydrogel composites: electron beam-initiated polymerization of layered cryogels.
Biomacromolecules. Roč. 16, č. 4 (2015), s. 1146-1156. ISSN 1525-7797. E-ISSN 1526-4602
R&D Projects: GA ČR(CZ) GA14-14961S; GA ČR(CZ) GAP108/11/1857; GA ČR(CZ) GA13-29009S; GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:61389013 ; RVO:61389005
Keywords : cryogels * mechanical properties * click chemistry
Subject RIV: CD - Macromolecular Chemistry; BG - Nuclear, Atomic and Molecular Physics, Colliders (UJF-V)
Impact factor: 5.583, year: 2015

The ability to tailor mechanical properties and architecture is crucial in creating macroporous hydrogel scaffolds for tissue engineering. In the present work, a technique for the modification of the pore size and stiffness of acrylamide-based cryogels is demonstrated via the regulation of an electron beam irradiation dose. The samples were characterized by equilibrium swelling measurements, light and scanning electron microscopy, mercury porosimetry, Brunauer–Emmett–Teller surface area analysis, and stiffness measurements. Their properties were compared to cryogels prepared by a standard redox-initiated radical polymerization. A 125I radiolabeled azidopentanoyl-GGGRGDSGGGY-NH2 peptide was bound to the surface to determine the concentration of the adhesive sites available for biomimetic modification. The functionality of the prepared substrates was evaluated by in vitro cultivation of adipose-derived stem cells. Moreover, the feasibility of preparing layered cryogels was demonstrated. This may be the key to the future preparation of complex hydrogel-based scaffolds to mimic the extracellular microenvironment in a wide range of applications.
Permanent Link: http://hdl.handle.net/11104/0246261