TRH-receptor mobility and function in intact and cholesterol-depleted plasma membrane of HEK293 cells stably expressing TRH-R-eGFP

0443142 - FGÚ 2016 RIV NL eng J - Journal Article
Brejchová, Jana - Sýkora, Jan - Ostašov, Pavel - Merta, Ladislav - Roubalová, Lenka - Janáček, Jiří - Hof, Martin - Svoboda, Petr
TRH-receptor mobility and function in intact and cholesterol-depleted plasma membrane of HEK293 cells stably expressing TRH-R-eGFP.
Biochimica Et Biophysica Acta-Biomembranes. Roč. 1848, č. 3 (2015), s. 781-796. ISSN 0005-2736. E-ISSN 1879-2642
R&D Projects: GA ČR(CZ) GAP207/12/0919
Institutional support: RVO:67985823 ; RVO:61388955
Keywords : cholesterol * TRH-R-eGFP mobility * FRAP * RICS * DPH fluorescence * G protein coupling
Subject RIV: CE - Biochemistry; CF - Physical ; Theoretical Chemistry (UFCH-W)
Impact factor: 3.687, year: 2015

Here we investigated the effect of disruption of plasma membrane integrity by cholesterol depletion on thyrotropin-releasing hormone receptor (TRH-R) surface mobility in HEK293 cells stably expressing TRH-R-eGFP fusion protein (VTGP cells). The apparent diffusion coefficient (Dapp) was decreased, but this decrease was detectable only under the special conditions of screening and calculation of FRAP data. Decrease of cholesterol level impaired the functional coupling between the receptor and the cognate G proteins of Gq/G11 family
Permanent Link: http://hdl.handle.net/11104/0245894