Molecular Characterization of Extrachromosomal DNA Accompanying Primula Red Phytoplasma

0443121 - BC 2016 RIV DE eng J - Journal Article
Sarkisova, Tatiana - Lenz, Ondřej - Petrzik, Karel
Molecular Characterization of Extrachromosomal DNA Accompanying Primula Red Phytoplasma.
Journal of Phytopathology - Phytopathologische Zeitschrift. Roč. 163, č. 3 (2015), s. 222-226. ISSN 0931-1785. E-ISSN 1439-0434
R&D Projects: GA MŠMT LD12074
Institutional support: RVO:60077344
Keywords : Phylogenetic analysis * phytoplasma * rep gene * plasmid
Subject RIV: EE - Microbiology, Virology
Impact factor: 0.945, year: 2015

The complete nucleotide sequence of an extrachromosomal element found in primula red isolate of Candidatus Phytoplasma asteris' (16SrI-B subgroup) was determined. The plasmid, named pPrR, is 4378bp in length and has 75% A+T content that is similar to that of the phytoplasma genome. It encodes six putative open reading frames (ORF) longer than 100 amino acids and two smaller ones. The structural organization of the rep gene is similar to that found in plasmids which replicate via rolling circle mechanism. Furthermore, it has homology to both the plasmid pLS1 family and helicase domains of replication-associated proteins (Rap) of eukaryotic viruses and geminiviruses. The ORF arrangement and genes sequences are most similar to the pPARG1 plasmid from Rehmannia glutinosa' phytoplasma.
Permanent Link: http://hdl.handle.net/11104/0245877