서식지에 따른 사철나무 잎의 이온화 방사선 조사 후 항산화 활성 변화 차이

0441010 - ÚEB 2015 RIV KP kor J - Journal Article
Kim, J. K. - Cha, M. - Wilhelmová, Naděžda - Mukherjee, A.
서식지에 따른 사철나무 잎의 이온화 방사선 조사 후 항산화 활성 변화 차이.
[Post-irradiation Changes in Antioxidant Activity of Spindle Tree Leaves from Different Areas.]
Korean Journal of Environmental Biology. Roč. 29, č. 4 (2011), s. 280-285. ISSN 1226-9999
R&D Projects: GA ČR(CZ) GAP501/11/1239
Institutional support: RVO:61389030
Keywords : stress * irradiation * antioxidants
Subject RIV: EF - Botanics
http://db.koreascholar.com/article.aspx?code=5556

l고등식물은 고정된 장소에 서식하기 때문에 주변 환경의 오염에 따른 부정적 영향을 회피할 수 없다. 대기 오염 지역과 청정지역에 서식하는 가로수의 이온화 방사선에 대한 효소활성 변화를 이용하여 대기오염 정도를 진단하는 방법을 개발하고자 본 연구를 수행하였다. 오염된 대기 속에 서식하며 대기에 포함된 오염물질로부터 지속적으로 산화스트레스를 받아온 식물체의 경우 이를 극복하기 위한 수단으로 생화학적 저항성을 발달시키게 된다. 항산화 효소와 자유라디칼 제거능.

As the plants grow in a fixed place, they can be a good indicator which reflects the level of environmental pollution. It is necessary for them to develop a strategy to cope with stress under unfavorable environmental conditions. In this study, spindle trees (Euonymus japonica) were collected from a clean area (Kijang) as well as a heavily polluted area (Onsan) to check applicability of irradiation combined with plant bioassay to environmental monitoring. The leaves were irradiated with 0, 50 and 100 Gy of gamma rays, and then evaluated for antioxidative capacity with 1,1- diphenyl-2-picrylhydrazyl (DPPH) assay and superoxide dismutase (SOD) analysis. The result shows that there was no significant changes in SOD and EDA (Electron Donationg Ability) in the samples collected from a polluted area. In the meanwhile, SOD increased in the samples from a clean area until 6 to 10 hours after irradiation, then it decreased gradually until 24 hours after irradiation. In conclusion, the plants in the polluted area have developed higher resistance to oxidative stress induced by ionizing radiation than those in the relatively clean area. Irradiation combined with plant bioassay on enzymatic activities and free radical scavenging capacity has proven to be a possible tool for biomonitoring the environmental pollution.
Permanent Link: http://hdl.handle.net/11104/0244071