Determining Omics Spatiotemporal Dimensions Using Exciting New Nanoscopy Techniques to Assess Complex Cell Responses to DNA Damage: Part B-Structuromics

0440773 - ÚJF 2015 RIV US eng J - Journal Article
Falk, Martin - Hausmann, M. - Lukášová, Emilie - Biswas, A. - Hildenbrand, G. - Davídková, Marie - Krasavin, E. - Kleibl, Z. - Falková, Iva - Ježková, L. - Štefančíková, Lenka - Ševčík, J. - Hofer, Michal - Bačíková, Alena - Matula, Pavel - Boreyko, A. - Vachelová, Jana - Jelínek Michaelidesová, Anna - Kozubek, Stanislav
Determining Omics Spatiotemporal Dimensions Using Exciting New Nanoscopy Techniques to Assess Complex Cell Responses to DNA Damage: Part B-Structuromics.
Critical Reviews in Eukaryotic Gene Expression. Roč. 24, č. 3 (2014), s. 225-247. ISSN 1045-4403. E-ISSN 2162-6502
R&D Projects: GA ČR GBP302/12/G157; GA ČR GAP302/10/1022; GA MŠMT LD12039; GA MŠMT LD12008; GA MŠMT(XE) LM2011019
Institutional support: RVO:68081707 ; RVO:61389005
Keywords : omics * ionizing radiation * low-dose dilemma * biological complexity and variability * higher-order chromatin structure * DNA damage response * formation of chromosomal translocations * confocal microscopy * localization nanoscopy
Subject RIV: BO - Biophysics; BO - Biophysics (BFU-R)
Impact factor: 1.571, year: 2014

n this Part B we show how high-resolution confocal microscopy as well as novel approaches of molecular localization nanoscopy fill the gaps to successfully place Omics data in the context of space and time. The dynamics of double-strand breaks during repair processes and chromosomal rearrangements at the microscale correlated to aberration induction are explained. For the first time we visualize pan-nuclear nucleosomal rearrangements and clustering at the nanoscale during repair processes. Finally, we introduce a novel method of specific chromatin nanotargeting based on a computer database search of uniquely binding oligonucleotide combinations (COMBO-FISH). With these challenging techniques on hand, we speculate future perspectives that may combine specific COMBO-FISH nanoprobing and structural nanoscopy to observe structure-function correlations in living cells in real-time. Thus, the Omics networks obtained from function analyses may be enriched by real-time visualization of Structuromics.
Permanent Link: http://hdl.handle.net/11104/0243900