Adsorptive Transfer Stripping for Quick Electrochemical Determination of microRNAs in Total RNA Samples

0440599 - BFÚ 2015 RIV DE eng J - Journal Article
Bartošík, M. - Hrstka, R. - Paleček, Emil - Vojtešek, B.
Adsorptive Transfer Stripping for Quick Electrochemical Determination of microRNAs in Total RNA Samples.
Electroanalysis. Roč. 26, č. 12 (2014), s. 2558-2562. ISSN 1040-0397. E-ISSN 1521-4109
R&D Projects: GA ČR(CZ) GA13-00956S
Institutional support: RVO:68081707
Keywords : Adsorptive transfer stripping * Cancer * Mercury electrode
Subject RIV: BO - Biophysics
Impact factor: 2.138, year: 2014

Electrochemical methods hold promise for fast and inexpensive determination of cancer biomarkers, including microRNAs playing an important role in regulation of gene expression. We developed a simple assay utilizing (i) microRNA labeling with osmium(VI) complexes, (ii) hybridization of microRNA with magnetic bead-immobilized capture probe, and (iii) adsorptive transfer stripping (AdTS) technique in which working electrode is dipped into a microliter-sized drop for direct adsorption of microRNA. Increased sensitivity and decreased sample consumption was achieved, and nanogram quantities of microRNA present in 500-fold excess of total RNA were determined. Our results indicate only negligible interference during hybridization from other RNA molecules.
Permanent Link: http://hdl.handle.net/11104/0243725