Characterization of the HMA7 gene and transcriptomic analysis of candidate genes for copper tolerance in two Silene vulgaris ecotypes

0440398 - BFÚ 2015 RIV PT eng J - Journal Article
Baloun, Jiří - Nevrtalová, Eva - Kováčová, Viera - Hudzieczek, Vojtěch - Čegan, Radim - Vyskot, Boris - Hobza, Roman
Characterization of the HMA7 gene and transcriptomic analysis of candidate genes for copper tolerance in two Silene vulgaris ecotypes.
Journal of Plant Physiology. Roč. 171, č. 13 (2014), s. 1188-1196. ISSN 0176-1617. E-ISSN 1618-1328
R&D Projects: GA ČR(CZ) GBP501/12/G090; GA MŠMT LO1204
Institutional support: RVO:68081707
Keywords : Copper * Genes coding ROS-eliminating and Cu-transporting proteins * RNA-Seq database
Subject RIV: BO - Biophysics
Impact factor: 2.557, year: 2014

Silene vulgaris possesses ecotype-specific tolerance to high levels of copper in the soil. Although this was reported a few decades ago, little is known about this trait on a molecular level. The aim of this study was to analyze the transcription response to elevated copper concentrations in two S. vulgaris ecotypes originating from copper-contrasting soil types - copper-tolerant Lubietova and copper-sensitive Stranska skala. To reveal if plants are transcriptionally affected, we first analyzed the HMA7 gene, a known key player in copper metabolism. Based on BAC library screening, we identified a BAC clone containing a SvHMA7 sequence with all the structural properties specific for plant copper-transporting ATPases. The functionality of the gene was tested using heterologous complementation in yeast mutants. Analyses of SvHMA7 transcription patterns showed that both ecotypes studied up-regulated SvHMA7 transcription after the copper treatment. Our data are supported by analysis of appropriate reference genes based on RNA-Seq databases.
Permanent Link: http://hdl.handle.net/11104/0243515