Temporal and spatial regulation of translation in the mammalian oocyte via the mTOR-eIF4F pathway

0440351 - ÚŽFG 2017 RIV GB eng J - Journal Article
Šušor, Andrej - Jansová, Denisa - Černá, Renata - Danylevska, Anna - Anger, Martin - Toralová, Tereza - Malík, Radek - Šupolíková, Jaroslava - Cook, M. S. - Oh, J. S. - Kubelka, Michal
Temporal and spatial regulation of translation in the mammalian oocyte via the mTOR-eIF4F pathway.
Nature Communications. Roč. 6, č. 6078 (2015). E-ISSN 2041-1723
R&D Projects: GA ČR GA13-12291S; GA ČR GAP502/12/2201; GA ČR GAP502/10/0944
Institutional support: RVO:67985904 ; RVO:68378050
Keywords : oocyte meiosis * localized in situ translation * mTOR
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 11.329, year: 2015

The fully-grown mammalian oocyte is transcriptionally quiescent and utilizes only transcripts synthesized and stored during early development. However, we find that an abundant RNA population is retained in the oocyte nucleus and contains specific mRNAs important for meiotic progression. During the first meiotic division, shortly after nuclear envelope breakdown, translational hotspots develop in the chromosomal area and in a region that previously surrounded the nucleus. These distinct translational hotspots are separated by endoplasmic reticulum and Lamin, and disappear following polar body extrusion. Chromosomal translational hotspots are controlled by the activity of the mTOR–eIF4F pathway. Here, we reveal a mechanism that—following the resumption of meiosis—controls the temporal and spatial translation of a specific set of transcripts required for normal spindle assembly, chromosome alignment and segregation.
Permanent Link: http://hdl.handle.net/11104/0243471